Publications
Research output from the Lau Laboratory
2024
Proteomics applications in next generation induced pluripotent stem cell models.
Manda, Vyshnavi , Pavelka, Jay , Lau, Edward
Journal Article Expert Rev Proteomics Vol. 21 (4 ) pp. 217-228 (2024)
INTRODUCTION: Induced pluripotent stem (iPS) cell technology has transformed biomedical research. New opportunities now exist to create new organoids, microtissues, and body-on-a-chip systems for basic biology investigations and clinical translations. …
Simultaneous proteome localization and turnover analysis reveals spatiotemporal features of protein homeostasis disruptions.
Currie, Jordan , Manda, Vyshnavi , Robinson, Sean K , Lai, Celine , Agnihotri, Vertica …
Journal Article Featured Nat Commun Vol. 15(1) pp. 2207 (2024)
The spatial and temporal distributions of proteins are critical to protein function, but cannot be directly assessed by measuring protein bundance. Here we describe a mass spectrometry-based proteomics strategy, Simultaneous …
Simultaneous proteome localization and turnover analysis reveals spatiotemporal features of protein homeostasis disruptions.
Currie, Jordan , Manda, Vyshnavi , Robinson, Sean K , Lai, Celine , Agnihotri, Vertica …
Preprint bioRxiv (2024)
The functions of proteins depend on their spatial and temporal distributions, which are not directly measured by static protein abundance. Under endoplasmic reticulum (ER) stress, the unfolded protein response (UPR) …
2023
Tissue Usage Preference and Intrinsically Disordered Region Remodeling of Alternative Splicing Derived Proteoforms in the Heart.
Pandi, Boomathi , Brenman, Stella , Black, Alexander , Ng, Dominic C M , Lau, …
Preprint bioRxiv (2023)
A computational analysis of mass spectrometry data was performed to uncover alternative splicing derived protein variants across chambers of the human heart. Evidence for 216 non-canonical isoforms was apparent in …
2022
Defining the Roles of Cardiokines in Human Aging and Age-Associated Diseases.
Srivastava, Himangi , Pozzoli, Marina , Lau, Edward
Journal Article Front Aging Vol. 3 pp. 884321 (2022)
In recent years an expanding collection of heart-secreted signaling proteins have been discovered that play cellular communication roles in diverse pathophysiological processes. This minireview briefly discusses current evidence for the …
Protein prediction models support widespread post-transcriptional regulation of protein abundance by interacting partners.
Srivastava, Himangi , Lippincott, Michael J , Currie, Jordan , Canfield, Robert , Lam, Maggie …
Journal Article PLoS Comput Biol Vol. 18 (11 ) pp. e1010702 (2022)
Protein and mRNA levels correlate only moderately. The availability of proteogenomics data sets with protein and transcript measurements from matching samples is providing new opportunities to assess the degree to …
Sex-specific responses to slow progressive pressure overload in a large animal model of HFpEF.
Eaton, Deborah M , Berretta, Remus M , Lynch, Jacqueline E , Travers, Joshua G …
Journal Article Am J Physiol Heart Circ Physiol Vol. 323 (4 ) pp. H797-H817 (2022)
Approximately 50% of all heart failure (HF) diagnoses can be classified as HF with preserved ejection fraction (HFpEF). HFpEF is more prevalent in females compared with males, but the underlying …
Proteogenomics reveals sex-biased aging genes and coordinated splicing in cardiac aging.
Han, Yu , Wennersten, Sara A , Wright, Julianna M , Ludwig, R W , …
Journal Article Am J Physiol Heart Circ Physiol Vol. 323 (3 ) pp. H538-H558 (2022)
The risks of heart diseases are significantly modulated by age and sex, but how these factors influence baseline cardiac gene expression remains incompletely understood. Here, we used RNA sequencing and …
Harmonizing Labeling and Analytical Strategies to Obtain Protein Turnover Rates in Intact Adult Animals.
Hammond, Dean E , Simpson, Deborah M , Franco, Catarina , Wright Muelas, Marina , …
Journal Article Mol Cell Proteomics Vol. 21 (7 ) pp. 100252 (2022)
Changes in the abundance of individual proteins in the proteome can be elicited by modulation of protein synthesis (the rate of input of newly synthesized proteins into the protein pool) …
Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammation.
Wei, Tzu-Tang , Chandy, Mark , Nishiga, Masataka , Zhang, Angela , Kumar, Kaavya Krishna …
Journal Article Cell Vol. 185 (10 ) pp. 1676-1693.e23 (2022)
Epidemiological studies reveal that marijuana increases the risk of cardiovascular disease (CVD); however, little is known about the mechanism. Delta(9)-tetrahydrocannabinol (Delta(9)-THC), the psychoactive component of marijuana, binds to cannabinoid receptor …
MiR-30 promotes fatty acid beta-oxidation and endothelial cell dysfunction and is a circulating biomarker of coronary microvascular dysfunction in pre-clinical models of diabetes.
Veitch, Shawn , Njock, Makon-Sebastien , Chandy, Mark , Siraj, M Ahsan , Chi, Lijun …
Journal Article Cardiovasc Diabetol Vol. 21 (1 ) pp. 31 (2022)
BACKGROUND: Type 2 diabetes (T2D) is associated with coronary microvascular dysfunction, which is thought to contribute to compromised diastolic function, ultimately culminating in heart failure with preserved ejection fraction (HFpEF). …
2021
Serum response factor deletion 5 regulates phospholamban phosphorylation and calcium uptake.
Woulfe, Kathleen C , Jeffrey, Danielle A , Pires Da Silva, Julie , Wilson, Cortney …
Journal Article J Mol Cell Cardiol Vol. 159 pp. 28-37 (2021)
AIMS: Pediatric dilated cardiomyopathy (pDCM) is characterized by unique age-dependent molecular mechanisms that include myocellular responses to therapy. We previously showed that pDCM, but not adult DCM patients respond to …
Transcriptome features of striated muscle aging and predictability of protein level changes.
Han, Yu , Li, Lauren Z , Kastury, Nikhitha L , Thomas, Cody T , …
Journal Article Mol Omics Vol. 17 (5 ) pp. 796-808 (2021)
We performed total RNA sequencing and multi-omics analysis comparing skeletal muscle and cardiac muscle in young adult (4 months) vs. early aging (20 months) mice to examine the molecular mechanisms …
Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot.
Han, Yu , Thomas, Cody T , Wennersten, Sara A , Lau, Edward , Lam, …
Journal Article J Vis Exp (2021)
Mass spectrometry-based shotgun proteomics experiments require multiple sample preparation steps, including enzymatic protein digestion and clean-up, which can take up significant person-hours of bench labor and present a source of …
Method for selective ablation of undifferentiated human pluripotent stem cell populations for cell-based therapies.
Chour, Tony , Tian, Lei , Lau, Edward , Thomas, Dilip , Itzhaki, Ilanit , …
Journal Article JCI Insight Vol. 6 (7 ) (2021)
Human pluripotent stem cells (PSCs), which are composed of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), provide an opportunity to advance cardiac cell therapy-based clinical trials. However, …
2020
Atlas of Exosomal microRNAs Secreted From Human iPSC-Derived Cardiac Cell Types.
Chandy, Mark , Rhee, June-Wha , Ozen, Mehmet O , Williams, Damon R , Pepic, …
Journal Article Circulation Vol. 142 (18 ) pp. 1794-1796 (2020)
Proteomic signatures of acute oxidative stress response to paraquat in the mouse heart.
Dostal, Vishantie , Wood, Silas D , Thomas, Cody T , Han, Yu , Lau, …
Journal Article Sci Rep Vol. 10 (1 ) pp. 18440 (2020)
The heart is sensitive to oxidative damage but a global view on how the cardiac proteome responds to oxidative stressors has yet to fully emerge. Using quantitative tandem mass spectrometry, …
High-throughput Preparation of DNA, RNA, and Protein from Cryopreserved Human iPSCs for Multi-omics Analysis.
Zhang, Jeffrey X , Lau, Edward , Paik, David T , Zhuge, Yan , Wu, …
Journal Article Curr Protoc Stem Cell Biol Vol. 54 (1 ) pp. e114 (2020)
We describe the procedure to isolate genomic DNA, RNA, and protein directly from cryopreserved induced pluripotent stem cell (iPSC) vials using commercially available solid-phase extraction kits, and we report the …
Modeling Secondary Iron Overload Cardiomyopathy with Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
Rhee, June-Wha , Yi, Hyoju , Thomas, Dilip , Lam, Chi Keung , Belbachir, Nadjet …
Journal Article Cell Rep Vol. 32 (2 ) pp. 107886 (2020)
Excessive iron accumulation in the heart causes iron overload cardiomyopathy (IOC), which initially presents as diastolic dysfunction and arrhythmia but progresses to systolic dysfunction and end-stage heart failure when left …
De novo mutations across 1,465 diverse genomes reveal mutational insights and reductions in the Amish founder population.
Kessler, Michael D , Loesch, Douglas P , Perry, James A , Heard-Costa, Nancy L …
Journal Article Proc Natl Acad Sci U S A Vol. 117 (5 ) pp. 2560-2569 (2020)
De novo mutations (DNMs), or mutations that appear in an individual despite not being seen in their parents, are an important source of genetic variation whose impact is relevant to …