Publications
Research output from the Lau Laboratory
2026
Subcellular spatiotemporal proteomics delineates distinct phases of ER stress proteostatic response.
Alamillo, Lorena , Paiz, Thalia , Black, Alexander , Juber, Matthew C , Arends, Ethan …
Journal Article bioRxiv (2026)
Endoplasmic reticulum (ER) stress is associated with many human diseases, but current understanding of how ER stress responses reshape the stressed proteome over time is still emerging. Specifically, how organellar …
Tracking cellular biomolecular condensate dynamics under proteostatic stress with middle-down phosphoproteomics.
Pandi, Boomathi , Ng, Dominic C M , Schaal, Peyton , Alamillo, Lorena , Binti, …
Journal Article bioRxiv (2026)
Coordination of biological function requires the partition of cellular components including into biomolecular condensates, but an overall landscape of how protein compartmentalize into higher-order assemblies under stress is still emerging. …
Micro- and Macro-Heterogeneity of N-Glycoproteome Remodeling in Endoplasmic Reticulum Stress.
Black, Alexander , Ngo, Tiffany , Pandi, Boomathi , Mongin, Andrew R , Ng, Dominic …
Journal Article Mol Cell Proteomics Vol. 25 (10 ) pp. 101653 (2026)
N-glycosylation plays essential roles in the folding, trafficking, and maturation of proteins in the secretory pathways, but how individual protein- and site-specific glycosylation rewires under endoplasmic reticulum (ER) stress is …
Body mass index-specific nanoparticle protein corona signatures in late pregnancy.
Velazquez, Samantha , Juber, Matthew C , Okun, Michele , Lau, Edward , Ashkarran, Ali …
Journal Article Nanoscale Vol. 18 (34 ) pp. 18571-18583 (2026)
The protein corona (PC) formed on the surface of nanoparticles (NPs) upon exposure to human biofluids is a dynamic interface that reflects the physiological and pathological status of the host. …
Proteogenomic analysis of the differential stability of cardiac protein isoforms.
Juber, Matthew , Binti, Shaonil , Pandi, Boomathi , Lau, Edward , Lam, Maggie P …
Journal Article bioRxiv (2026)
Alternative splicing is an important regulatory layer in gene expression, but knowledge on the isoform protein molecules continue to lag their canonical counterparts. An open question is whether alternative protein …
Accelerated Turnover of Collagens and Other Extracellular Matrix Proteins Upon Chronic Beta-Adrenergic Stimulation.
Juber, Matthew C , Cox, Jacob G , Paiz, Thalia , McKinsey, Timothy A , …
Journal Article JACC Basic Transl Sci Vol. 11 (7 ) pp. 101594 (2026)
Consensus statement on mass spectrometry-based proteomic analysis of cardiac tissue.
Lundby, Alicia , Van Eyk, Jennifer E , Mayr, Manuel , White, Melanie Y , …
Journal Article Nat Cardiovasc Res (2026)
Mass spectrometry-based cardiac proteomics provides direct molecular insight into cardiac physiology and disease. While plasma proteomics has advanced biomarker discovery, the analysis of cardiac tissue is essential for mechanistic understanding …
Body Mass Index-Specific Nanoparticle Protein Corona Signatures in Late Pregnancy.
Velazquez, Samantha , Juber, Matthew C , Okun, Michele , Lau, Edward , Ashkarran, Ali …
Journal Article bioRxiv (2026)
The protein corona (PC) formed on the surface of nanoparticles (NPs) upon exposure to human biofluids is a dynamic interface that reflects the physiological and pathological status of the host. …
MXene-Protein Corona Interfaces for Molecular Profiling of Alzheimer's Disease.
Velazquez, Samantha , Juber, Matthew C , Brindley, Dalton , Thakur, Anupma , Anasoori, Babak …
Journal Article bioRxiv (2026)
The protein corona (PC) that forms on the surface of nanomaterials upon contact with biological fluids provides a molecular snapshot of the host's physiological and pathological state. Here, we investigate …
Protocol to measure protein half-life in cell culture using heavy water.
Alamillo, Lorena , Black, Alexander , Juber, Matthew C , Ng, Dominic C M , …
Journal Article STAR Protoc Vol. 7(1) pp. 104426 (2026)
Protein turnover, comprising the continued synthesis and clearance of proteins, is required for protein homeostasis and cell survival. Here, we present a protocol to measure protein turnover half-life in cultured …
Longevity of cardiac and skeletal muscle proteins is dependent on tissue and subcellular compartmentation patterns.
Gugel, Jack , Currie, Jordan , Alamillo, Lorena , Flint, Jason , Kim, Keun-Young , …
Journal Article Cell Rep Vol. 45(1) pp. 116768 (2026)
Myocytes are exceptionally long-lived cells that must maintain proteome integrity over decades while adjusting for changes in functional output and metabolic demand. We used in vivo stable isotope labeling combined …
2025
SALVE: prediction of interorgan communication with transcriptome latent space representation.
Pavelka, Jay , Voong, Calvin K , Schaal, Peyton , Lam, Maggie P Y , …
Journal Article Featured Am J Physiol Heart Circ Physiol Vol. 329(6) pp. H1621-H1632 (2025)
Massive transcriptomics data allow gene relationships to be discovered from their correlated expression. We describe secretome association with latent variables between tissues (SALVE), a method to infer the associations between …
A ratiometric catalog of protein isoform shifts in the cardiac fetal gene program.
Han, Yu , Binti, Shaonil , Wennersten, Sara A , Pandi, Boomathi , Ng, Dominic …
Journal Article JCI Insight Vol. 10 (18 ) (2025)
Pathological cardiac remodeling is associated with the reactivation of fetal genes, yet the extent of the heart's fetal gene program and its impact on proteome compositions remain incompletely understood. Here, …
Protein Turnover Dynamics Analysis With Subcellular Spatial Resolution.
Alamillo, Lorena , Black, Alexander , Lam, Maggie P Y , Lau, Edward
Journal Article Bio Protoc Vol. 15 (15 ) pp. e5409 (2025)
Protein synthesis and degradation (i.e., turnover) forms an important part of protein homeostasis and has been implicated in many age- associated diseases. Different cellular locations, such as organelles and membraneless …
Deuterium labeling enables proteome-wide turnover kinetics analysis in cell culture.
Alamillo, Lorena , Ng, Dominic C M , Currie, Jordan , Black, Alexander , Pandi, …
Journal Article Featured Cell Rep Methods Vol. 5(7) pp. 101104 (2025)
Protein turnover is a critical component of gene expression regulation and cellular homeostasis, yet methods for measuring turnover rates that are scalable and applicable to different models are still needed. …
A Ratiometric Catalog of Protein Isoform Shifts in the Cardiac Fetal Gene Program.
Han, Yu , Binti, Shaonil , Wennersten, Sara A , Pandi, Boomathi , Ng, Dominic …
Journal Article bioRxiv (2025)
Pathological cardiac remodeling is associated with the reactivation of fetal genes, yet the extent of the heart's fetal gene program and its impact on proteome compositions remain incompletely understood. Here, …
Improved Method to Determine Protein Turnover Rates with Heavy Water Labeling by Mass Isotopomer Ratio Selection.
Currie, Jordan , Ng, Dominic C M , Pandi, Boomathi , Black, Alexander , Manda, …
Journal Article J Proteome Res Vol. 24 (4 ) pp. 1992-2005 (2025)
The synthesis and degradation rates of proteins form an essential component of gene expression control. Heavy water labeling has been used in conjunction with mass spectrometry to measure protein turnover …
Deuterium labeling enables proteome wide turnover kinetics analysis in cell culture.
Alamillo, Lorena , Ng, Dominic C M , Currie, Jordan , Black, Alexander , Pandi, …
Journal Article bioRxiv (2025)
The half-life of proteins is tightly regulated and underlies many cellular processes. It remains unclear the extent to which proteins are dynamically synthesized and degraded in different cell types and …
2024
Tissue Usage Preference and Intrinsically Disordered Region Remodeling of Alternative Splicing Derived Proteoforms in the Heart.
Pandi, Boomathi , Brenman, Stella , Black, Alexander , Ng, Dominic C M , Lau, …
Journal Article J Proteome Res Vol. 23 (8 ) pp. 3161-3173 (2024)
A computational analysis of mass spectrometry data was performed to uncover alternative splicing derived protein variants across chambers of the human heart. Evidence for 216 non-canonical isoforms was apparent in …
Improved determination of protein turnover rate with heavy water labeling by mass isotopomer ratio selection.
Currie, Jordan , Ng, Dominic C M , Pandi, Boomathi , Black, Alexander , Manda, …
Journal Article bioRxiv (2024)
The synthesis and degradation rates of proteins form an essential component of gene expression control. Heavy water labeling has been used in conjunction with mass spectrometry to measure protein turnover …