Protein turnover and spatiotemporal dynamics in health and disease
We are interested in the regulation and function of protein turnover, homeostasis, and secretion in development, senescence, and diseases. Our work leverages advances in proteomics, bioinformatics, and human induced pluripotent stem cell (iPSC) models. Two current areas of focus are:
What is the role of protein turnover and homeostasis during cell fate transitions?Our lab develops analytical and computational methods that can measure the individual turnover rates and half-life of thousands of protein species in complex systems. These techniques have been used to reveal changes in protein synthesis and degradation in animal models and discover new disease signatures. A current focus is to understand the regulation and function of protein quality control and proteolysis during cellular differentiation, stress, and senescence in iPSC systems.
How do cells in the body communicate through secreted RNAs and proteins?In recent work, we have mapped secreted non-coding RNAs from multiple cell types derived from human iPSCs (cardiomyocytes, endothelial cells, fibroblasts) that may function in intercellular communications and that may be harnessed as a quantitative metric to assess the differentiation status and purity of hPSC-derived cardiac cells. In ongoing work, we are leveraging this approach to model the longitudinal changes in cellular communication networks under stress and disease using a combination of computational modeling and proteomics strategies.
Work in the laboratory is supported by funding from an NIH/NHLBI R00 award, NIH/OD R03 award, as well as the University of Colorado Consortium for Fibrosis Research & Translation.
Current Position: PhD Student at CU Anschutz Integrated Physiology
Current Position: PhD Student at CU Anschutz Bioengineering
Protocol to extract biomolecules from cryopreserved hiPSC vials published in Current Protocol.