Cardiac Secretome

Background: Exosomes and other extracellular vesicles released from the heart have drawn recent interest for their potential function in paracrine communications and regenerative therapies. This dataset contains the RNA-seq abundance of secreted the exosomal small RNAs including microRNA and piwi-interacting RNAs released from human induced pluripotent stem cells (hiPSCs) as well as hiPSC-derived cardiac cell types (cardiomyocytes, endothelial cells, and fibroblasts).


Methods

At a Glance

120

Number of miRNAs

216

Number of piRNAs

12

Number of Samples

Frequently Asked Questions

In the small RNA dataset, we consider a molecule to be secreted at an appreciable level if the average quantile-normalized read counts in both biological replicates are 10 or above. For a secreted molecule to be considered enriched in a cell type, it must also have 5-fold or higher read counts in a single tested cell type compared to the other three cell types in the experiment.

To determine tissue expression patterns, we considered the median total/intracellular miRNA levels in 17 human tissues (spleen, myocardium, small intestine, stomach, bone, brain cerebral cortex frontal, brain cerebellum, liver, gallbladder, bladder, lung, testis, colon, muscle, pancreas, esophagus, prostate, thyroid) using a previously published dataset on human miRNA tissue distributions (Ludwig et al. 2016).

We currently have data from miRNA and piRNA from small RNA sequencing. Other data types including exosomal proteins will be added in the future.

Lau Lab

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